Mouse Hyperinsulinemic - Euglycemic Clamp

Our pumps, swivels and tethers are standard equipment for mouse glucose clamp experiments. Both the infusion and sampling lines are run through a 375/D/25LT low-torque two-channel swivel connected to a two-channel mouse Vascular Access Button™. The infusion line is connected to a jugular vein catheter, the sampling line to a carotid artery catheter. While the surgery to place two catheters is difficult, collecting blood outside the cage from a freely-moving animal, rather than from the tail from a restrained animal, minimizes stress which will impact the data.

The Vascular Access Button™ permits quick, aseptic connection and disconnection of the IV lines, and also allows the mice to be group housed when they are not on study. The VABM2T/25GCY16 tether uses a looped wire to connect to the swivel so that the lines are visible, and includes a Y connector built-in to the button connector for tracer injections. 

This configuration is based on the clamp technique used and taught by the Vanderbilt MMPC, with the VAB™ replacing a researcher-constructed MASA device. If you need help with the surgery you can take in-person courses at Vanderbilt, The Jackson Laboratory or RRSSC, or live online courses from Instech.

Watch a video demonstration of mouse glucose clamp surgery and equipment.