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Rat Bile Collection
Instech's two- and three-channel Vascular Access Buttons and Harnesses are at the heart of elegant systems for rodent bile collection. Install the button or harness at the same time that catheters are placed in the bile duct and duodenum. No special occlusion catheters are needed. (A single C33PU-RBD1302 catheter can be used for both bile duct and duodenum.) Attach a loop connector so that bile flow, now diverted through the harness, can resume in an extracorporeal loop during recovery and transport. Begin collecting bile by attaching a tether attached to a swivel. There are a range of options depending on whether bile salts need to be replaced or not, and whether a third catheter is needed for blood sampling or infusion. (See PDF below.)
The components listed below allow bile collection outside the cage with replacement bile salts delivered on a second channel with a syringe pump (A.3.).
systems information [PDF]
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